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  Syringe and ampoule
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  using an ampoule
  using a multidose vial


Self-administration using an ampoule

Please note that these instructions are for guidance only. Speak to your doctor or fertility specialist for full directions on self-administration.

Mixing (or reconstituting) ampoules

  • Wash your hands thoroughly
  • Place materials on a dry, clean surface
  • Hold the powder ampoule upright with the dot facing away from you
  • Using a gauze, grasp the top of the ampoule between your thumb and index finger. 
    • Carefully snap the neck of the ampoule by twisting outward with the tips of your thumbs.    

  • Remove the protective cap from the syringe, being careful not to touch the needle. 
    • If you choose to use the long (40mm) needle to withdraw sterile water, hold the capped portion while placing onto the nozzle of the syringe.     

  • Insert the needle into the sterile water and pull back the plunger to the 1 ml mark, being careful not to remove the plunger.
  • Insert the sterile water into an ampoule of powder by pressing down on the plunger and aiming at the side of the ampoule, not directly onto the powder. 
    • Do not shake the ampoule. 
    • If bubbles appear, wait a few moments for the bubbles to settle.
    • Use only if the mix is clear and colourless.     

  • If your dose requires more than one ampoule:
    • Draw the mixture back up into the syringe. 
    • Be sure to keep the tip of the needle within the mixture while withdrawing.
    • Then add the contents of the syringe to the next ampoule of powder. 
    • This process should be repeated until your recommended dose is completely mixed.    


Preparing your dose



  • Your fertility team will instruct you on your exact dose.
  • If you used a 40mm needle for mixing, replace it with a short 12mm needle.
  • Carefully cover the long needle with a cap. 
  • Next, grasp the barrel of the syringe, pull off the covered needle and discard it in the safety container.
  • Place the short needle into the nozzle of the syringe and remove the needle cap. 
    • Remember not to touch the needle.   

  • Turn it so the needle is pointing up, and tap the syringe. 
  • After all the bubbles float to the top, lightly press the plunger until a drop or two of liquid is released from the tip of the needle. 
  • Next, put the protective cap back on the needle while you prepare the injection site.
Injecting your dose

  • Your doctor or nurse will have advised you where to inject (abdomen, thigh or upper arm). 
  • Swab the injection site with alcohol and allow it to dry.
  • Carefully pull the needle cap from the syringe. 
  • Holding the syringe in one hand, hold the skin around the injection site.
  • Insert the needle at a slight angle.
  • Press down on the plunger in a slow, steady motion until all the medication is injected. 
  • Withdraw the needle and dispose of it in your sharps container.
  • If any bleeding occurs, apply gauze to the site with gentle pressure.


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Last Updated: 5/6/2008

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